defmodule Bio.IO.Fasta do @moduledoc """ Allow the input/output of FASTA formatted files. Reads and writes fasta data to and from a number of formats. The FASTA file format is composed of pairs of lines where the pair is demarcated by the ">" character. All data proceeding the ">" character represents the 'header' of the pair, while the next line after a newline represents sequence data. The FASTA file format does not specify the type of the data in the sequence. That means that you can reasonably store RNA, DNA, amino acid, or realistically any other polymer sequence using the format. In general, the expectation is that the data is ASCII encoded. """ @type header :: String.t() @type sequence :: String.t() @type read_opts :: {:type, any()} | {:parse_header, (String.t() -> String.t())} @type fasta_data :: [String.t()] | [struct()] | [{header(), sequence()}] | %{headers: [header()], sequences: [sequence()]} @doc """ Read a FASTA formatted file The `read/2` function returns an error tuple of the content or error code from `File.read`. You can use `:file.format_error/1` to get a descriptive string of the error. You can specify the return type of the contents by using a module which matches the `Bio.Behaviours.Sequence`. Specifically the type must have a `new/2` method that matches the spec of the behaviour. ## Options - `:type` - The module for the type of struct you wish to have returned. This should minimally implement a `new/2` function equivalent to the `Bio.Behaviours.Sequence` behaviour. - `:parse_header` - A callable for parsing the header values of the FASTA file. """ @spec read(filename :: Path.t(), opts :: [read_opts]) :: {:ok, any()} | {:error, File.posix()} def read(filename, opts \\ []) do type = Keyword.get(opts, :type, Bio.Sequence) h_fn = Keyword.get(opts, :parse_header, & &1) case File.read(filename) do {:ok, content} -> {:ok, parse(content, "", [], :header, type, h_fn)} not_ok -> not_ok end end @doc """ Read a FASTA formatted file The same as `read/2`, but will raise a `File.Error` on failure. """ @spec read!(filename :: Path.t(), opts :: [read_opts]) :: any() | no_return() def read!(filename, opts \\ []) do type = Keyword.get(opts, :type) h_fn = Keyword.get(opts, :parse_header, & &1) {:ok, parse(File.read!(filename), "", [], :header, type, h_fn)} end @doc """ Write a FASTA file using sequence data. The data type that this function accepts is varied. Help with whatever your workflow requires, the `List` types are: List: ``` elixir [{header(), sequence()}, ...] [header(), sequence(), header(), sequence(), ...] [%Bio.Sequence._{}, ...] ``` Where `%Bio.Sequence._{}` indicates any struct of the `Bio.Sequence` module or child modules implementing the `Bio.Behaviours.Sequence` behaviour. It also supports data in a `Map` format: ``` elixir %{ headers: [header(), ...], sequences: [sequence(), ...] } ``` ## Examples iex> Fasta.write("/tmp/test_file.fasta", ["header", "sequence", "header2", "sequence2"]) :ok Will return error types in common with `File.write/3` """ @spec write(filename :: Path.t(), data :: fasta_data, [File.mode()]) :: :ok | {:error, File.posix()} def write(filename, data, modes \\ []) def write(filename, {header, sequence}, modes) do File.write(filename, ">#{header}\n#{sequence}\n", modes) end def write(filename, [header, sequence], modes) do File.write(filename, ">#{header}\n#{sequence}\n", modes) end def write(filename, data, modes) when is_list(data) do [datum | _] = data data = if is_binary(datum) do data |> Enum.chunk_every(2) else data end data |> Enum.reduce("", &to_line/2) |> then(fn output -> File.write(filename, output, modes) end) end def write(filename, %{headers: headers, sequences: sequences}, modes) do Enum.zip(sequences, headers) |> Enum.reduce("", &to_line/2) |> then(fn output -> File.write(filename, output, modes) end) end defp parse(content, value, acc, _ctx, type, header_fn) when content == "" do # this will be [seq, header] for all the parsed seqs [value | acc] |> Enum.chunk_every(2) |> Enum.reduce([], fn [seq, header], acc -> List.insert_at(acc, 0, apply(type, :new, [seq, [label: header_fn.(header)]])) end) end defp parse(content, value, acc, ctx, type, header_fn) when ctx == :header do <> = content case char do ">" -> parse(rest, value, acc, :header, type, header_fn) "\n" -> parse(rest, "", [value | acc], :sequence, type, header_fn) _ -> parse(rest, value <> char, acc, :header, type, header_fn) end end defp parse(content, value, acc, ctx, type, header_fn) when ctx == :sequence do <> = content case char do ">" -> parse(rest, "", [value | acc], :header, type, header_fn) "\n" -> parse(rest, value, acc, :sequence, type, header_fn) _ -> parse(rest, value <> char, acc, :sequence, type, header_fn) end end defp to_line([sequence, header], acc) do acc <> ">#{header}\n#{sequence}\n" end defp to_line({sequence, header}, acc) do acc <> ">#{header}\n#{sequence}\n" end defp to_line(%_{} = datum, acc) do acc <> apply(datum.__struct__, :fasta_line, [datum]) end defmodule Binary do @moduledoc false @doc false def new(sequence, label: label), do: {sequence, label} end end